diff --git a/mkdocs/docs/reference/env.md b/mkdocs/docs/reference/env.md
index 4a169fc22f..c721de395c 100644
--- a/mkdocs/docs/reference/env.md
+++ b/mkdocs/docs/reference/env.md
@@ -132,7 +132,7 @@ For more details on the options below, refer to the [server deployment](../guide
 - `DSTACK_OTEL_METRICS_ENABLED`{ #DSTACK_OTEL_METRICS_ENABLED } – Enables OpenTelemetry metrics if set to any value. Requires the `otel` extra.
 - `DSTACK_OTEL_METRICS_EXPORTERS`{ #DSTACK_OTEL_METRICS_EXPORTERS } – A comma-separated list of OpenTelemetry metrics exporters: `otlp` (push via OTLP) and/or `prometheus` (expose on the `/metrics` endpoint). Defaults to `otlp`.
 - `DSTACK_DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE`{ #DSTACK_DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE } – Request body size limit for services running with a gateway, in bytes. Defaults to 64 MiB.
-- `DSTACK_SERVICE_CLIENT_TIMEOUT`{ #DSTACK_SERVICE_CLIENT_TIMEOUT } – Timeout in seconds for HTTP requests sent from the in-server proxy and gateways to service replicas. Defaults to 60.
+- `DSTACK_SERVICE_CLIENT_TIMEOUT`{ #DSTACK_SERVICE_CLIENT_TIMEOUT } – How long (in seconds) the in-server proxy and gateways wait for a service replica to send data. Defaults to 300.
 - `DSTACK_FORBID_SERVICES_WITHOUT_GATEWAY`{ #DSTACK_FORBID_SERVICES_WITHOUT_GATEWAY } – Forbids registering new services without a gateway if set to any value.
 - `DSTACK_FORBID_DSTACK_IN_RUNS`{ #DSTACK_FORBID_DSTACK_IN_RUNS } – Forbids submitting runs with `dstack: true` (dstack server access inside runs) if set to any value.
 - `DSTACK_SERVER_CODE_UPLOAD_LIMIT`{ #DSTACK_SERVER_CODE_UPLOAD_LIMIT } - The repo size limit when uploading diffs or local repos, in bytes. Set to `0` to disable size limits. Defaults to `2MiB`.
diff --git a/src/dstack/_internal/proxy/gateway/models.py b/src/dstack/_internal/proxy/gateway/models.py
index 3e0a76c032..80ce0b8abe 100644
--- a/src/dstack/_internal/proxy/gateway/models.py
+++ b/src/dstack/_internal/proxy/gateway/models.py
@@ -4,6 +4,7 @@
 
 from pydantic import AnyHttpUrl
 
+from dstack._internal.proxy.lib.const import DEFAULT_SERVICE_READ_TIMEOUT
 from dstack._internal.proxy.lib.models import ImmutableModel
 
 
@@ -11,6 +12,7 @@ class ModelEntrypoint(ImmutableModel):
     project_name: str
     domain: str
     https: bool
+    read_timeout: int = DEFAULT_SERVICE_READ_TIMEOUT
 
 
 class ACMESettings(ImmutableModel):
diff --git a/src/dstack/_internal/proxy/gateway/resources/nginx/entrypoint.jinja2 b/src/dstack/_internal/proxy/gateway/resources/nginx/entrypoint.jinja2
index bcb66e1dd4..ce0160f558 100644
--- a/src/dstack/_internal/proxy/gateway/resources/nginx/entrypoint.jinja2
+++ b/src/dstack/_internal/proxy/gateway/resources/nginx/entrypoint.jinja2
@@ -4,7 +4,7 @@ server {
         proxy_pass http://localhost:{{ proxy_port }}/api/models/{{ project_name }}/;
         proxy_set_header X-Real-IP $remote_addr;
         proxy_set_header Host $host;
-        proxy_read_timeout 300s;
+        proxy_read_timeout {{ read_timeout }}s;
     }
     listen 80;
     {% if https %}
diff --git a/src/dstack/_internal/proxy/gateway/resources/nginx/service.jinja2 b/src/dstack/_internal/proxy/gateway/resources/nginx/service.jinja2
index 4f2adda389..e3eced5f6d 100644
--- a/src/dstack/_internal/proxy/gateway/resources/nginx/service.jinja2
+++ b/src/dstack/_internal/proxy/gateway/resources/nginx/service.jinja2
@@ -68,7 +68,7 @@ server {
         proxy_http_version 1.1;
         proxy_set_header Upgrade $http_upgrade;
         proxy_set_header Connection "Upgrade";
-        proxy_read_timeout 300s;
+        proxy_read_timeout {{ read_timeout }}s;
         {% else %}
         return 503;
         {% endif %}
@@ -88,7 +88,7 @@ server {
         proxy_pass http://{{ domain }}.upstream;
         proxy_set_header X-Real-IP $remote_addr;
         proxy_set_header Host $host;
-        proxy_read_timeout 300s;
+        proxy_read_timeout {{ read_timeout }}s;
         {% if not proxy_buffering %}
         proxy_buffering off;
         {% endif %}
diff --git a/src/dstack/_internal/proxy/gateway/routers/registry.py b/src/dstack/_internal/proxy/gateway/routers/registry.py
index c5fb7c0d6a..954ae2933a 100644
--- a/src/dstack/_internal/proxy/gateway/routers/registry.py
+++ b/src/dstack/_internal/proxy/gateway/routers/registry.py
@@ -35,6 +35,7 @@ async def register_service(
         rate_limits=body.rate_limits,
         auth=body.auth,
         client_max_body_size=body.client_max_body_size,
+        read_timeout=body.read_timeout,
         model=body.options.openai.model if body.options.openai is not None else None,
         ssh_private_key=body.ssh_private_key,
         repo=repo,
@@ -139,6 +140,7 @@ async def register_entrypoint(
         project_name=project_name.lower(),
         domain=body.domain.lower(),
         https=body.https,
+        read_timeout=body.read_timeout,
         repo=repo,
         nginx=nginx,
     )
diff --git a/src/dstack/_internal/proxy/gateway/schemas/registry.py b/src/dstack/_internal/proxy/gateway/schemas/registry.py
index 9a800c2ceb..25f801252a 100644
--- a/src/dstack/_internal/proxy/gateway/schemas/registry.py
+++ b/src/dstack/_internal/proxy/gateway/schemas/registry.py
@@ -3,6 +3,7 @@
 from pydantic import BaseModel, Field
 
 from dstack._internal.core.models.instances import SSHConnectionParams
+from dstack._internal.proxy.lib.const import DEFAULT_SERVICE_READ_TIMEOUT
 from dstack._internal.proxy.lib.models import RateLimit
 
 
@@ -47,6 +48,7 @@ class RegisterServiceRequest(BaseModel):
     ssh_private_key: str
     rate_limits: tuple[RateLimit, ...] = ()
     has_router_replica: bool = False
+    read_timeout: int = DEFAULT_SERVICE_READ_TIMEOUT
 
 
 class SetServiceIdRequest(BaseModel):
@@ -68,3 +70,4 @@ class RegisterReplicaRequest(BaseModel):
 class RegisterEntrypointRequest(BaseModel):
     domain: str
     https: bool
+    read_timeout: int = DEFAULT_SERVICE_READ_TIMEOUT
diff --git a/src/dstack/_internal/proxy/gateway/services/nginx.py b/src/dstack/_internal/proxy/gateway/services/nginx.py
index 1486ea0c22..df71594f5d 100644
--- a/src/dstack/_internal/proxy/gateway/services/nginx.py
+++ b/src/dstack/_internal/proxy/gateway/services/nginx.py
@@ -69,11 +69,13 @@ class ServiceConfig(SiteConfig):
     has_router_replica: bool = False
     cors_enabled: bool = False
     proxy_buffering: bool = True
+    read_timeout: int
 
 
 class ModelEntrypointConfig(SiteConfig):
     type: Literal["entrypoint"] = "entrypoint"
     project_name: str
+    read_timeout: int
 
 
 class Nginx:
diff --git a/src/dstack/_internal/proxy/gateway/services/registry.py b/src/dstack/_internal/proxy/gateway/services/registry.py
index c69b57136a..2ce9ea9ffd 100644
--- a/src/dstack/_internal/proxy/gateway/services/registry.py
+++ b/src/dstack/_internal/proxy/gateway/services/registry.py
@@ -42,6 +42,7 @@ async def register_service(
     rate_limits: tuple[models.RateLimit, ...],
     auth: bool,
     client_max_body_size: int,
+    read_timeout: int,
     model: Optional[schemas.AnyModel],
     ssh_private_key: str,
     repo: GatewayProxyRepo,
@@ -63,6 +64,7 @@ async def register_service(
         has_router_replica=has_router_replica,
         cors_enabled=cors_enabled,
         proxy_buffering=model is None,
+        read_timeout=read_timeout,
     )
 
     async with lock:
@@ -252,6 +254,7 @@ async def register_model_entrypoint(
     project_name: str,
     domain: str,
     https: bool,
+    read_timeout: int,
     repo: GatewayProxyRepo,
     nginx: Nginx,
 ) -> None:
@@ -259,6 +262,7 @@ async def register_model_entrypoint(
         project_name=project_name,
         domain=domain,
         https=https,
+        read_timeout=read_timeout,
     )
     logger.debug("Registering entrypoint %s in project %s", domain, project_name)
     await apply_entrypoint(entrypoint, repo, nginx)
@@ -409,6 +413,7 @@ async def get_nginx_service_config(
         has_router_replica=service.has_router_replica,
         cors_enabled=service.cors_enabled,
         proxy_buffering=service.proxy_buffering,
+        read_timeout=service.read_timeout,
     )
 
 
@@ -419,6 +424,7 @@ async def apply_entrypoint(
         domain=entrypoint.domain,
         https=entrypoint.https,
         project_name=entrypoint.project_name,
+        read_timeout=entrypoint.read_timeout,
     )
     acme = (await repo.get_config()).acme_settings
     await nginx.register(config, acme)
diff --git a/src/dstack/_internal/proxy/lib/const.py b/src/dstack/_internal/proxy/lib/const.py
index 43ede03ac8..0824a5edb7 100644
--- a/src/dstack/_internal/proxy/lib/const.py
+++ b/src/dstack/_internal/proxy/lib/const.py
@@ -2,6 +2,8 @@
 Shared constants for proxy components (gateway + in-server proxy).
 """
 
+DEFAULT_SERVICE_READ_TIMEOUT = 300
+
 # Inference endpoints exposed by the in-replica HTTP router. Applies to both
 # SGLang's router and Dynamo's `dynamo.frontend` — they share the
 # OpenAI-compatible endpoint surface.
diff --git a/src/dstack/_internal/proxy/lib/models.py b/src/dstack/_internal/proxy/lib/models.py
index 511f27d70c..44d5878bbd 100644
--- a/src/dstack/_internal/proxy/lib/models.py
+++ b/src/dstack/_internal/proxy/lib/models.py
@@ -7,6 +7,7 @@
 from typing_extensions import Annotated
 
 from dstack._internal.core.models.instances import SSHConnectionParams
+from dstack._internal.proxy.lib.const import DEFAULT_SERVICE_READ_TIMEOUT
 from dstack._internal.proxy.lib.errors import UnexpectedProxyError
 
 
@@ -66,6 +67,8 @@ class Service(ImmutableModel):
     proxy_buffering: bool = True
     """Only used on gateways. Disabled for services with a model so that streamed responses
     reach the client as the replica emits them."""
+    read_timeout: int = DEFAULT_SERVICE_READ_TIMEOUT
+    """Seconds to wait for data from a replica between two successive reads."""
 
     @model_validator(mode="before")
     @classmethod
diff --git a/src/dstack/_internal/proxy/lib/services/service_connection.py b/src/dstack/_internal/proxy/lib/services/service_connection.py
index 37bdc5083a..c8229ad53a 100644
--- a/src/dstack/_internal/proxy/lib/services/service_connection.py
+++ b/src/dstack/_internal/proxy/lib/services/service_connection.py
@@ -19,14 +19,11 @@
 from dstack._internal.proxy.lib.models import Project, Replica, Service
 from dstack._internal.proxy.lib.repo import BaseProxyRepo
 from dstack._internal.utils.common import get_or_error
-from dstack._internal.utils.env import environ
 from dstack._internal.utils.logging import get_logger
 from dstack._internal.utils.path import FileContent
 
 logger = get_logger(__name__)
 OPEN_TUNNEL_TIMEOUT = 10
-HTTP_TIMEOUT = environ.get_int("DSTACK_SERVICE_CLIENT_TIMEOUT", default=60)
-# Same as default Nginx proxy timeout; override via DSTACK_SERVICE_CLIENT_TIMEOUT
 
 
 class ServiceClient(httpx.AsyncClient):
@@ -75,7 +72,7 @@ def __init__(self, project: Project, service: Service, replica: Replica) -> None
             # The hostname in base_url is there for troubleshooting, as it may appear in
             # logs and in the Host header. The actual destination is the Unix socket.
             base_url=f"http://{replica.id}-{service.run_name}/",
-            timeout=HTTP_TIMEOUT,
+            timeout=service.read_timeout,
         )
         self._is_open = asyncio.locks.Event()
 
@@ -148,7 +145,7 @@ async def get_service_replica_client(
         return httpx.AsyncClient(
             base_url="http://127.0.0.1",
             headers={"Host": service.domain},
-            timeout=HTTP_TIMEOUT,
+            timeout=service.read_timeout,
         )
     # Nginx not available, forward directly to the tunnel
     replica = random.choice(service.replicas)
diff --git a/src/dstack/_internal/server/background/pipeline_tasks/gateway_replicas.py b/src/dstack/_internal/server/background/pipeline_tasks/gateway_replicas.py
index 44fd687cda..7e51f90eff 100644
--- a/src/dstack/_internal/server/background/pipeline_tasks/gateway_replicas.py
+++ b/src/dstack/_internal/server/background/pipeline_tasks/gateway_replicas.py
@@ -1288,6 +1288,7 @@ async def _register_service(
         ),
         auth=run_spec.configuration.auth,
         client_max_body_size=settings.DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE,
+        read_timeout=settings.SERVICE_CLIENT_TIMEOUT,
         options=service_spec.options,
         rate_limits=run_spec.configuration.rate_limits,
         ssh_private_key=run_model.project.ssh_private_key,
diff --git a/src/dstack/_internal/server/services/gateways/client.py b/src/dstack/_internal/server/services/gateways/client.py
index ed6c8d78b9..0c9defecca 100644
--- a/src/dstack/_internal/server/services/gateways/client.py
+++ b/src/dstack/_internal/server/services/gateways/client.py
@@ -45,6 +45,7 @@ async def register_service(
         gateway_https: bool,
         auth: bool,
         client_max_body_size: int,
+        read_timeout: int,
         options: dict,
         rate_limits: list[RateLimit],
         ssh_private_key: str,
@@ -52,7 +53,7 @@ async def register_service(
     ):
         if "openai" in options:
             entrypoint = f"gateway.{domain.split('.', maxsplit=1)[1]}"
-            await self.register_openai_entrypoint(project, entrypoint, gateway_https)
+            await self.register_openai_entrypoint(project, entrypoint, gateway_https, read_timeout)
 
         payload = {
             "id": run_id.hex,
@@ -61,6 +62,7 @@ async def register_service(
             "https": service_https,
             "auth": auth,
             "client_max_body_size": client_max_body_size,
+            "read_timeout": read_timeout,
             "options": options,
             "rate_limits": [limit.model_dump() for limit in rate_limits],
             "ssh_private_key": ssh_private_key,
@@ -160,12 +162,15 @@ async def set_service_id(self, project: str, run_name: str, run_id: uuid.UUID) -
         resp.raise_for_status()
         self.is_server_ready = True
 
-    async def register_openai_entrypoint(self, project: str, domain: str, https: bool):
+    async def register_openai_entrypoint(
+        self, project: str, domain: str, https: bool, read_timeout: int
+    ):
         resp = await self._client.post(
             self._url(f"/api/registry/{project}/entrypoints/register"),
             json={
                 "domain": domain,
                 "https": https,
+                "read_timeout": read_timeout,
             },
         )
         if resp.status_code == 400:
diff --git a/src/dstack/_internal/server/services/proxy/repo.py b/src/dstack/_internal/server/services/proxy/repo.py
index d0afd41def..7ea7df43cd 100644
--- a/src/dstack/_internal/server/services/proxy/repo.py
+++ b/src/dstack/_internal/server/services/proxy/repo.py
@@ -32,7 +32,10 @@
 from dstack._internal.server.services.instances import get_instance_remote_connection_info
 from dstack._internal.server.services.jobs import get_job_spec
 from dstack._internal.server.services.runs import get_run_spec
-from dstack._internal.server.settings import DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE
+from dstack._internal.server.settings import (
+    DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE,
+    SERVICE_CLIENT_TIMEOUT,
+)
 from dstack._internal.utils.common import get_or_error
 
 _ANY_MODEL_ADAPTER = pydantic.TypeAdapter(AnyModel)
@@ -136,6 +139,7 @@ async def get_service(self, project_name: str, run_name: str) -> Optional[Servic
             strip_prefix=run_spec.configuration.strip_prefix,
             replicas=tuple(replicas),
             has_router_replica=has_router_replica,
+            read_timeout=SERVICE_CLIENT_TIMEOUT,
         )
 
     async def list_models(self, project_name: str) -> List[ChatModel]:
diff --git a/src/dstack/_internal/server/settings.py b/src/dstack/_internal/server/settings.py
index efe6e320fc..c077feb4ea 100644
--- a/src/dstack/_internal/server/settings.py
+++ b/src/dstack/_internal/server/settings.py
@@ -180,6 +180,7 @@ def get_database_url() -> str:
 DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE = int(
     os.getenv("DSTACK_DEFAULT_SERVICE_CLIENT_MAX_BODY_SIZE", 64 * 1024 * 1024)
 )
+SERVICE_CLIENT_TIMEOUT = environ.get_int("DSTACK_SERVICE_CLIENT_TIMEOUT", default=300)
 
 SERVER_DEFAULT_DOCKER_REGISTRY = os.getenv("DSTACK_SERVER_DEFAULT_DOCKER_REGISTRY") or None
 SERVER_DEFAULT_DOCKER_REGISTRY_USERNAME = (
diff --git a/src/tests/_internal/proxy/gateway/routers/test_registry.py b/src/tests/_internal/proxy/gateway/routers/test_registry.py
index 48c7a41d5f..393b901acc 100644
--- a/src/tests/_internal/proxy/gateway/routers/test_registry.py
+++ b/src/tests/_internal/proxy/gateway/routers/test_registry.py
@@ -31,8 +31,9 @@ def register_service_payload(
     client_max_body_size: int = 1024,
     options: Optional[dict] = None,
     rate_limits: Optional[list[dict]] = None,
+    read_timeout: Optional[int] = None,
 ) -> dict:
-    return {
+    payload = {
         "id": uuid.uuid4().hex,
         "run_name": run_name,
         "domain": domain,
@@ -43,6 +44,9 @@ def register_service_payload(
         "rate_limits": rate_limits or [],
         "ssh_private_key": "private-key",
     }
+    if read_timeout is not None:
+        payload["read_timeout"] = read_timeout
+    return payload
 
 
 def register_replica_payload(job_id: str = "xxx-xxx") -> dict:
@@ -234,6 +238,26 @@ async def test_register_with_model(self, tmp_path: Path, system_mocks: Mocks) ->
         conf = (tmp_path / "sites-enabled" / "443-test-run.gtw.test.conf").read_text()
         assert "proxy_buffering off;" in conf
 
+    async def test_register_with_read_timeout(self, tmp_path: Path, system_mocks: Mocks) -> None:
+        repo = GatewayProxyRepo()
+        client = make_client(tmp_path, repo=repo)
+        resp = await client.post(
+            "/api/registry/test-proj/services/register",
+            json=register_service_payload(run_name="test-run", read_timeout=900),
+        )
+        assert resp.status_code == 200
+        service = await repo.get_service("test-proj", "test-run")
+        assert service is not None
+        assert service.read_timeout == 900
+        resp = await client.post(
+            "/api/registry/test-proj/services/test-run/replicas/register",
+            json=register_replica_payload(),
+        )
+        assert resp.status_code == 200
+        conf = (tmp_path / "sites-enabled" / "443-test-run.gtw.test.conf").read_text()
+        assert conf.count("proxy_read_timeout 900s;") == 2
+        assert "proxy_read_timeout 300s;" not in conf
+
     async def test_register_with_rate_limits(self, tmp_path: Path, system_mocks: Mocks) -> None:
         client = make_client(tmp_path)
         resp = await client.post(
@@ -599,6 +623,19 @@ async def test_register(self, tmp_path: Path, system_mocks: Mocks) -> None:
         assert system_mocks.reload_nginx.call_count == 1
         assert system_mocks.run_certbot.call_count == 0
 
+    async def test_register_with_read_timeout(self, tmp_path: Path, system_mocks: Mocks) -> None:
+        repo = GatewayProxyRepo()
+        client = make_client(tmp_path, repo=repo)
+        resp = await client.post(
+            "/api/registry/test-proj/entrypoints/register",
+            json={"domain": "gateway.gtw.test", "https": False, "read_timeout": 900},
+        )
+        assert resp.status_code == 200
+        conf = (tmp_path / "sites-enabled" / "443-gateway.gtw.test.conf").read_text()
+        assert "proxy_read_timeout 900s;" in conf
+        [entrypoint] = await repo.list_entrypoints()
+        assert entrypoint.read_timeout == 900
+
     async def test_register_with_https(self, tmp_path: Path, system_mocks: Mocks) -> None:
         client = make_client(tmp_path)
         resp = await client.post(
diff --git a/src/tests/_internal/server/background/pipeline_tasks/test_gateway_replicas.py b/src/tests/_internal/server/background/pipeline_tasks/test_gateway_replicas.py
index 07f0a00b5f..aaddd0b7eb 100644
--- a/src/tests/_internal/server/background/pipeline_tasks/test_gateway_replicas.py
+++ b/src/tests/_internal/server/background/pipeline_tasks/test_gateway_replicas.py
@@ -20,6 +20,7 @@
 from dstack._internal.core.models.instances import InstanceStatus
 from dstack._internal.core.models.runs import JobStatus, RunStatus, ServiceSpec
 from dstack._internal.proxy.gateway.schemas.services import ServiceListItem, ServiceListReplicaItem
+from dstack._internal.server import settings
 from dstack._internal.server.background.pipeline_tasks.gateway_replicas import (
     GatewayReplicaFetcher,
     GatewayReplicaPipeline,
@@ -817,7 +818,9 @@ async def test_registers_new_service_and_replica(
         session: AsyncSession,
         worker: GatewayReplicaWorker,
         mock_gateway_connection: AsyncMock,
+        monkeypatch: pytest.MonkeyPatch,
     ):
+        monkeypatch.setattr(settings, "SERVICE_CLIENT_TIMEOUT", 900)
         project = await create_project(session=session)
         user = await create_user(session=session)
         repo = await create_repo(session=session, project_id=project.id)
@@ -858,6 +861,7 @@ async def test_registers_new_service_and_replica(
             gateway_https=ANY,
             auth=ANY,
             client_max_body_size=ANY,
+            read_timeout=900,
             options={},
             rate_limits=[],
             ssh_private_key=project.ssh_private_key,
@@ -1432,6 +1436,7 @@ async def test_unregisters_and_reregisters_legacy_service_without_id_and_replica
             gateway_https=ANY,
             auth=ANY,
             client_max_body_size=ANY,
+            read_timeout=ANY,
             options={},
             rate_limits=[],
             ssh_private_key=project.ssh_private_key,